My company's main agent products: human elisa kit, rat ELISA kit, mouse ELISA kit, serum, antibodies, biological research reagents. There are typically two antigen-antibody reactions in an ELISA kit, ie, after adding the sample and adding the enzyme conjugate. The completion of the antigen-antibody reaction requires a certain temperature and time. This incubation process is called incubation, which is called incubation and may not be appropriate in an ELISA kit. ELISA is a solid phase immunoassay in which antigen and antibody binding occurs only on the surface of a solid phase. Taking the sandwich method of antibody coating as an example, the specimen added to the well of the plate does not have an equal opportunity to bind to the solid phase, and only the antigen in the solution closest to the pore wall is directly in contact with the antibody. . This is a process of gradual equilibrium, so it needs to be diffused to reach the end of the reaction. The same applies to the binding of the enzyme-labeled antibody added thereafter to the solid phase antigen. This is why ELISA reactions always require a certain amount of incubation. The temperatures commonly used for incubation are 43 ° C, 37 ° C, room temperature and 4 ° C. 37 ° C is the usual incubation temperature in the laboratory, and is also the appropriate temperature for most antigen-antibody binding. When the ELISA method was established as the reaction kinetics study, the experiment showed that the two antigen-antibody reactions generally reached the peak at 37 ° C for 1 to 2 hours. In order to accelerate the reaction, the temperature of the reaction can be raised. Some tests are carried out at 43 ° C, but higher temperatures are not suitable. The antigen-antibody reaction was more thorough at 4 ° C, and the reaction was mostly allowed to stand overnight in the refrigerator in a radioimmunoassay to form the most precipitate. However, because it takes too long, it is generally not used in ELISA kits. Insulation method In addition to the special ELISA instrument with special electric heating block, the water bath is generally used. The ELISA kit plate can be placed in the water bath box. The bottom of the ELISA plate should be attached to the water surface to make the temperature balance quickly. In order to avoid evaporation, the board should be covered, or the plate hole can be covered with plastic sealing paper or plastic wrap. At this time, the reaction plate can float on the water surface. If an incubator is used, the ELISA kit should be placed in a wet box. The wet box should be made of a material with good heat transfer such as metal, a wet gauze at the bottom of the box, and finally the ELISA plate should be placed on the wet gauze. The wet box should be pre-warmed in the incubator to the specified temperature, especially at lower temperatures. Whether it is water bath or wet box incubation, the reaction plates should not be stacked to ensure that the temperature of each plate can be quickly balanced. For the reaction at room temperature, the room temperature during the operation should be strictly limited to the specified range. The standard room temperature refers to 20 to 25 ° C, but the specific conditions can be controlled according to the requirements of the instructions. When incubated at room temperature, the ELISA kit can be placed flat on the console. It should be noted that the temperature and time of incubation should be as accurate as required. In order to ensure this, one person should not measure more than two plates at the same time. Shanghai Jinma ELISA Kit suppliers distribute wholesale ELISA kits, serum, antibodies, biological reagents, standards, Omega kits, radioimmunoassay kits, culture media, laboratory consumables, and cells to the consumer market. With a high status, the company has established long-term and stable cooperative relationships with many retailers and agents. Shanghai Hengyuan elisa kit suppliers distribute ELISA kits, serum, antibodies, biological reagents, standards, Omega kits, radioimmunoassay kits, culture media, laboratory consumables, complete cell varieties, and reasonable prices. Shanghai Jinma ELISA kit suppliers have strong strength, re-credit, abide by the contract, guarantee product quality, and have won the trust of customers with the principle of multi-variety management and the principle of small profits but quick turnover.